Ultraviolet (UV) light is widely used for disinfection across various industries, including water treatment, air quality control, and facility maintenance. But not all UV technologies perform the same.
Two leading technologies in this space—254nm UV-C and pulsed xenon—use fundamentally different approaches. Pulsed xenon produces intense bursts of broad-spectrum light, while 254nm UV-C delivers a steady, focused wavelength optimized for microbial inactivation.
So, which one performs better in real-world environments?
In this article, we break down why 254nm UV-C is considered the gold standard in UV disinfection—offering superior efficacy, consistency, safety, and operational practicality.
Where does UV light come from?

UV light is a type of electromagnetic radiation that falls between visible light and X-rays on the spectrum. It is categorized into three primary ranges:
• UV-A (315–400 nm): The longest wavelength, often associated with material degradation and surface-level reactions.
• UV-B (280–315 nm): More energetic than UV-A and capable of affecting organic tissue, but less efficient for targeted disinfection.
• UV-C (200–280 nm): The most energetic and effective range for inactivating microorganisms by damaging their genetic material.
Unlike UV-A and UV-B, UV-C does not naturally reach Earth’s surface—it’s absorbed by the ozone layer. To use UV-C for disinfection, we rely on artificial sources like low-pressure mercury lamps and UV-C LEDs.
Why is 254nm so important?

The 254nm wavelength falls within the UV-C range and is the most efficiently absorbed by microbial DNA and RNA. When exposed to this precise wavelength, microorganisms experience the formation of pyrimidine dimers—types of genetic disruption that interfere with their ability to replicate.
This mechanism makes 254nm UV-C highly effective for microbial inactivation.
What is pulsed xenon?
Pulsed xenon is a disinfection technology that generates quick, high-intensity flashes of broad-spectrum light. These pulses are produced by sending electrical energy through xenon gas, emitting light across ultraviolet (UV), visible, and infrared wavelengths.
Comparing the germicidal efficacy of 254nm UV-C vs. pulsed xenon
A common misconception is that using a wide range of wavelengths across the UV light spectrum–UV-A, UV-B, and UV-C–results in better disinfection than using a single, targeted wavelength. At first glance, it may seem logical to assume that combining multiple wavelengths would enhance overall effectiveness. However, in UV disinfection, wavelength energy, absorption, and exposure time determine germicidal efficacy–not wavelength variety.
254nm UV-C sits at the peak absorption point for microbial genetic material, delivering the precise energy needed to inactivate microorganisms effectively. In contrast, pulsed xenon systems emit mostly UV-A and UV-B, with only a small portion of output falling within the germicidal UV-C range—meaning much of the light is outside the optimal zone for disinfection.
Additionally, an independent study published in 2019 found that systems using 254nm UV-C achieved significantly higher levels of microbial inactivation than those using pulsed xenon.
We break this down further in this short video that debunks the “more wavelengths = better disinfection” myth.
The importance of consistent UV output
One of 254nm UV-C’s biggest advantages is its steady, uninterrupted light output. This consistent emission ensures even exposure across all surfaces during the disinfection cycle—reducing the risk of untreated areas.
Pulsed xenon, by contrast, operates in rapid bursts. This stop-and-go approach increases the chance of uneven UV coverage, leaving some surfaces with insufficient exposure and potentially limiting effectiveness.
The safety risks of pulsed xenon

A major reason 254nm UV-C is the preferred choice in many settings is its ability to be fully contained within enclosed systems. It doesn’t penetrate glass or small gaps, such as door sills, making it safer for targeted use.
In contrast, pulsed xenon poses more safety challenges. Since it emits UV-A and UV-B, it can pass through glass and small openings, increasing the risk of unintended exposure.
To reduce this risk, operators are often required to hang blackout curtains—adding time, labor, and complexity to every disinfection cycle.
Additionally, pulsed xenon emits wavelengths below 240nm, which can generate ozone—a hazardous gas that can cause respiratory irritation or health concerns with prolonged exposure.
254nm UV-C integrates seamlessly into daily operations
Another key advantage of 254nm UV-C is its ability to operate quietly and consistently within day-to-day routines. Its steady output and minimal operational footprint make it well-suited for environments where reliability and workflow continuity are essential.
By contrast, pulsed xenon can be more disruptive. Each burst generates a loud pop or thump, and the intense flashes of light may cause discomfort for nearby individuals. These interruptions can limit its practicality in many real-world settings.
Final verdict: Why 254nm UV-C is the gold standard
When comparing the two technologies, 254nm UV-C consistently outperforms pulsed xenon across all key metrics:
- Higher efficacy – 254nm UV-C is precisely tuned for microbial inactivation.
- Steady, continuous output – Ensures reliable disinfection without gaps in coverage.
- Safer containment – No blackout curtains needed, and no ozone generation.
- More practical operation – Runs quietly and fits easily into existing protocols.
For facilities looking for a powerful, efficient, and low-maintenance disinfection solution, 254nm UV-C stands out as the smarter choice over pulsed xenon.